Journal: Molecular Vision

Article Title: Effect of curcumin on the modulation of αA- and αB-crystallin and heat shock protein 70 in selenium-induced cataractogenesis in Wistar rat pups

doi:

Figure Lengend Snippet: Effects of curcumin on immunohistochemistry of αA- and αB-crystallin in the eye lens of Wistar rat pups exposed to selenium. A : This image shows the control lens incubated in saline alone without any antibody treatment. B : This is a control lens that was incubated with saline and subjected to antibody treatment. C : Lens from rat pups administered with selenium alone. D : Lens from rat pups administered with selenium and curcumin simultaneously. E : Lens from rat pups administered with selenium first and then treated with curcumin after 24 h. F : Lens from rat pups pretreated with curcumin and then administered with selenium after 24 h. Lens sections were preincubated with αA- and αB-crystallin polyclonal antirabbit Immunoglobulin G (IgG) antibody (1:3,000 dilution) and subsequently with goat anti-rabbit IgG-horse radish peroxidase (HRP) conjugate (1:3,000 dilution). The immunoreactivity was developed with 0.01% 3,3-diaminobenzidine tetrahydrochloride (DAB) and H 2 O 2 . Note the brown color formation indicative of peroxidase reaction in the nucleus. Image G , negative control, lens treated with goat anti-rabbit IgG-HRP and developed using DAB and hydrogen peroxide (H 2 O 2 ). The figure shows the high level of αA- and αB-crystallin expression induced by selenium-mediated oxidative stress. This increased crystallin expression and aggregate formation was prevented by curcumin pretreatment.

Article Snippet: After washing with TBS containing 0.05% Tween 20, the sections were incubated with the primary antibody, αA- and αB-crystallin polyclonal anti-rabbit IgG antibody (BD Biosciences, San Jose, CA), at a dilution of 1:500 overnight at 4 °C.

Techniques: Immunohistochemistry, Incubation, Negative Control, Expressing

Journal: Molecular Vision

Article Title: Effect of curcumin on the modulation of αA- and αB-crystallin and heat shock protein 70 in selenium-induced cataractogenesis in Wistar rat pups

doi:

Figure Lengend Snippet: Immunoblot expression of αA- and αB-crystallin in control and experimental group of animals. Lane I, eye lens protein from control (physiologic saline) rat pups (group I); lane II, eye lens protein from selenium-injected rat pups (group II); lane III, eye lens protein from rat pups administered selenium and curcumin simultaneously (group III); lane IV, eye lens protein from rat pups injected with selenium 24 h before being administered with curcumin (group IV); and lane V, eye lens protein from rat pups administered with curcumin 24 h before being injected with selenium (group V). The separated lens protein was preincubated with αA- and αB-crystallin polyclonal antirabbit IgG antibody (1:3,000 dilution) and subsequently with goat antirabbit IgG-HRP (1:3,000 dilution). The immunoreactivity was developed with 0.01% DAB and H 2 O 2 . β-Actin refers to house keeping protein expression and its levels are constant across all treatment groups indicating the normal behaviour of lenses under various treatment. The figure clearly shows increased αA- and αB-crystallin protein expression under selenium-mediated oxidative stress. This increased crystallin protein expression was prevented by curcumin pretreatment.

Article Snippet: After washing with TBS containing 0.05% Tween 20, the sections were incubated with the primary antibody, αA- and αB-crystallin polyclonal anti-rabbit IgG antibody (BD Biosciences, San Jose, CA), at a dilution of 1:500 overnight at 4 °C.

Techniques: Western Blot, Expressing, Injection

Journal: bioRxiv

Article Title: Specificity and effector functions of non-neutralizing gB-specific monoclonal antibodies isolated from healthy individuals with human cytomegalovirus infection

doi: 10.1101/2020.01.14.907204

Figure Lengend Snippet: gB-specific mAbs mediate negligible NK activation ADCC responses when compared to polyclonal CMV-specific antibody preparation (Cytogam). gB-specific mAbs were screened for mediation of NK cell (A) CD107a degranulation and (B) CD16 downregulation (DRI) as measures of antibody-mediated NK cell activation. Data are presented as mAb specific response against AD169r-GFP infected ARPE19 cells minus mock infected negative control cells.

Article Snippet: Purity and identity were confirmed by Western blot using polyclonal CMV IgG (Cytogam – CSL Behring) or monoclonal antibodies SM10 (Domain I) and SM5-1 (Domain II).

Techniques: Activation Assay, Infection, Negative Control